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mmp 3  (R&D Systems)


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    R&D Systems mmp 3
    Mmp 3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mmp+3/Human+MMP+Premixed+Magnetic+Luminex%C2%AE+Performance+Assay/pm41917896-101-19-23
    Average 92 stars, based on 7 article reviews
    mmp 3 - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Recombinant:

    Article Title: C-terminal CDNF and MANF fragments, pharmaceutical compositions comprising same and uses thereof
    Article Snippet: .. Recombinant Human MMP-2, MMP-3, MMP-9, and elastase-2 proteins are purchased, for example, from R&D Systems. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Fluoxetine Reshapes Macrophage Membrane Sphingolipids and Inflammatory Response Without Affecting Extracellular Vesicle Biogenesis upon Inactivated SARS-CoV-2 Stimulation.
    Article Snippet: For the morphological assessment, EV suspensions were adsorbed onto carbon-coated Transmission Electron Microscopy (TEM) grids (Electron Microscopy Sciences, Hatfield, PA, USA) for 20 min, fixed with 1% glutaraldehyde (Sigma-Aldrich, St. Louis, MO, USA), rinsed with deionized water (MilliQ, Darmstadt, Germany), and visualized using a JEM-2100 microscope (JEOL, Tokyo, Japan), available at Electron Microscopy Multiuser Laboratory at Department of Cell and Molecular Biology (FMRP-USP). .. Secretion of IL-6 (Cat. No. 555157), IL-1β (Cat. No. 557953), MMP-3 (Cat. No. DY513), and MMP-9 (Cat. No. DY911) in culture supernatants was quantified using BD OptEIATM Human ELISA kits for cytokines (BD Biosciences, Franklin Lakes, NJ, USA) and RD DuoSetHuman ELISA kits for MMPs (R&D System, Minneapolis, MN, USA), Supplementary Table S1, following the manufacturers’ protocol. .. Data visualization and statistical comparisons were performed in GraphPad Prism (v9.1, San Diego, CA, USA).

    Article Title: Fluoxetine Reshapes Macrophage Membrane Sphingolipids and Inflammatory Response Without Affecting Extracellular Vesicle Biogenesis upon Inactivated SARS-CoV-2 Stimulation
    Article Snippet: For the morphological assessment, EV suspensions were adsorbed onto carbon-coated Transmission Electron Microscopy (TEM) grids (Electron Microscopy Sciences, Hatfield, PA, USA) for 20 min, fixed with 1% glutaraldehyde (Sigma-Aldrich, St. Louis, MO, USA), rinsed with deionized water (MilliQ, Darmstadt, Germany), and visualized using a JEM-2100 microscope (JEOL, Tokyo, Japan), available at Electron Microscopy Multiuser Laboratory at Department of Cell and Molecular Biology (FMRP-USP). .. Secretion of IL-6 (Cat. No. 555157), IL-1β (Cat. No. 557953), MMP-3 (Cat. No. DY513), and MMP-9 (Cat. No. DY911) in culture supernatants was quantified using BD OptEIATM Human ELISA kits for cytokines (BD Biosciences, Franklin Lakes, NJ, USA) and RD DuoSet-Human ELISA kits for MMPs (R&D System, Minneapolis, MN, USA), , following the manufacturers’ protocol. .. Data visualization and statistical comparisons were performed in GraphPad Prism (v9.1, San Diego, CA, USA).



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    Image Search Results


    Hsa_circ_0101645 accelerating the IVDD process in vivo . A: Diagram of the animal procedure for this study. B: Grouping information for this section. C: Representative X-rays of each group of rats. Statistical graph demonstrating the disc height index (DHI) changes for L4/5 in each group of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). D: HE staining exhibiting pathological changes of CEP, NP, and AP in IVD in each group of rats (N = 6). Scale bar: 500 μm. E: EdU staining was used to detect cell proliferation in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. F: TUNEL (white light) staining exhibiting TUNEL-positive cells in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. G: The effect of hsa_circ_0101645 on the protein levels of Collagen Ⅱ, Aggrecan, MMP-3 and MMP-13 in IVD was observed by IHC staining (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 100 μm. H-I: The expression of hsa_circ_0101645 (H) and miR-1304-5p (I) in each group of IVD tissues (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). J-K: Changes in expression of apoptosis ( J; Caspase 3, Bcl-2 and Bax) and autophagy markers ( K; LC3B, Beclin and P62) in IVD tissues (N = 3) (One-way ANOVA test with Tukey's multiple comparisons test or Kruskal-Wallis test with Dunn's multiple comparisons test). ∗ indicates P < 0.05.

    Journal: Non-coding RNA Research

    Article Title: Hsa_circ_0101645 contributes to excessive autophagy and apoptosis in intervertebral disc degeneration by acting as a miR-1304-5p sponge modulating BNIP3 expression

    doi: 10.1016/j.ncrna.2025.11.007

    Figure Lengend Snippet: Hsa_circ_0101645 accelerating the IVDD process in vivo . A: Diagram of the animal procedure for this study. B: Grouping information for this section. C: Representative X-rays of each group of rats. Statistical graph demonstrating the disc height index (DHI) changes for L4/5 in each group of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). D: HE staining exhibiting pathological changes of CEP, NP, and AP in IVD in each group of rats (N = 6). Scale bar: 500 μm. E: EdU staining was used to detect cell proliferation in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. F: TUNEL (white light) staining exhibiting TUNEL-positive cells in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. G: The effect of hsa_circ_0101645 on the protein levels of Collagen Ⅱ, Aggrecan, MMP-3 and MMP-13 in IVD was observed by IHC staining (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 100 μm. H-I: The expression of hsa_circ_0101645 (H) and miR-1304-5p (I) in each group of IVD tissues (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). J-K: Changes in expression of apoptosis ( J; Caspase 3, Bcl-2 and Bax) and autophagy markers ( K; LC3B, Beclin and P62) in IVD tissues (N = 3) (One-way ANOVA test with Tukey's multiple comparisons test or Kruskal-Wallis test with Dunn's multiple comparisons test). ∗ indicates P < 0.05.

    Article Snippet: Sections were then incubated with primary antibodies against Collagen II (28459-1-AP; 1:200; Proteintech, USA), Aggrecan (13880-1-AP; 1:100; Proteintech), MMP-3 (17873-1-AP; 1:200; Proteintech), and MMP-13 (18165-1-AP; 1:100; Proteintech), followed by the corresponding secondary antibodies.

    Techniques: In Vivo, Staining, TUNEL Assay, Immunohistochemistry, Expressing